Title Using vaginal cytology to assess the estrogenic activity of phytoestrogen-rich herb
Author Suchinda Malaijitnond a,*, Kullakanya Chansri a, Pisamai Kijkuokul b, Nontakorn Urasopon c, Wichai Cherdshewasart a
Subject Using vaginal cytology to assess the estrogenic activity of phytoestrogen-rich herb
Institute of Research
Date Issue
Keyword Keywords: Pueraria mirifica; Pueraria lobata; Phytoestrogens; Vaginal cytology assay; Ovariectomized rats
Abstract

Abstract: To assess the estrogenic activities of synthetic estrogen, synthetic phytoestrogen, Pueraria lobata and three distinct cultivars of Pueraria mirifica, a phytoestrogen-rich herb, a vaginal cytology assay in ovariectomized rats were used. Rats were ovariectomized and treated with DW, estradiol valerate (1 mg/kg BW), genistein (0.25-2.5 mg/kg BW), Pueraria lobata and Pueraria mirifica (10-1000 mg/kg BW) for 14 days. The vaginal cytology was checked daily and the uteri were dissected and weighed at the end of treatment or post-treatment periods. The treatments of DW, genistein and Pueraria lobata did not influence the vaginal epithelium, but the injection valerate induced a vaginal cornification from day-3 of treatment to day-14 of post-treatment period. The occurrence of vaginal cornification after treatment and the recovery after the cessation was dependent on dosages and cultivars of Pueraria mirifica. The increments of uterus weight in all rats agreed with the cornification of vaginal epithelium. Although both uterotropic and vaginal cytology assays can be used to assess the estrogenic activity of phytoestrogen-rich herb, however, using vaginal cytology assay has two advantages : (1) we do not need to kill the animals and (2) we can follow up the recovery after the cessation of treatment. © 2006 Elsevier Ireland Ltd. All rights reserved. Keywords: Pueraria mirifica; Pueraria lobata; Phytoestrogens; Vaginal cytology assay; Ovariectomized rats

Title A study of the action of miroestrol and other oestrogens on the reproductive tract of the immature female mouse
Author By H.E.H. JONES AND G.S. POPE
Subject A study of the action of miroestrol and other oestrogens on the reproductive tract of the immature female mouse
Institute of Research
Date Issue
Keyword
Abstract

SUMMARY 1. Assay of the potency of the recently isolated plant oestrogen, miroestrol, shows that when given subcutaneously in multiple doses it is as potent as oestrodiol-17 â, and orally more than three time as potent as stilboestrol in producing an increase in uterine weight in the immature female mouse. 2. In the immature female mouse given a single injection of oestrogens, the route of administration influence the magnitude, the latent period, and the duration of the response in the reproductive tract. In general, the oestrogen act most efficiently on the uterus and vagina when they are given intraperitoncally instead of subcutaneously. It is suggested that this is because there is less chance of absorption rates and metabolism in the animal influencing the oestrogen introduced intraperitoncally. 3. The activity of miroestrol by single injection is described and compared with that of oestriol, oestrodiol-17 â and stilboestrol. It is at least as effective as oestrodiol-17 â and stilboestrol by prerasting uterine and vaginal growth, and in increasing the amount of fluid which can be expressed from the uterine lumen by gentle pressing.