Title Toxicity Tests of a Phytoestrogen-Rich herb; Pueraria mirifica
Author Department of Biology, Faculty of Science, Chulalongkom University,
Subject
Institute of Research
Date Issue
Keyword
Abstract ..More Infomation

A study to determine the toxicity of the powder and extract derived from the Phytoestrogen-rich herb Pueraria Mirifica Cultvan Wichai-III was carred out Using a selection of anmals and some human volunteers. The powder was used to determine the acute toxicity mimice with a. 0.5 dilution criterion . It was found that the ED50 of the oral consumption of the powder was out of the range of 2,000 mg/kg

b.w. A skin mtation test was perfomed on rabbits with 1 ml/head epidermal application. The rabbits showed no signs of irritation symptoms. A.Draze test was performed on rabbits along with an eye irritation test. No irritation was observed up to the end of the 7th day of the test period. A skin irritation test was conducted on guinea pigs by applying GPMT to assess contact allergic response It was scrod as a no allergic response . The extract treated skin showed now Allergic response after UV-A tradiation during the photo-toxicity test on gunea pigs. The primary human skin test using an applid Draize test revealed no meaningful allergic response. From all established toxicity tests it may be concluded that P. mirifica cultivar Wichai-III should be outside any significance acute toxicology range if orally consumed or should not cause any significance skin sensitization if topically applied

Title Efficacy and Safety of Pueraria mirifica (Kwao Kruea Khao) for the Treatment of Vasomotor Symptoms in Premenopausal Women: Phase II Study
Author SURACHAI LAMLERTKITTIKUL, MD*,VERAPOL CHANDEYING, MD**
Subject Efficacy and Safety of Pueraria mirifica (Kwao Kruea Khao) for the Treatment of Vasomotor Symptoms in Premenopausal Women: Phase II Study
Institute of Research
Date Issue
Keyword
Abstract

Objectives : To evaluate the preliminary efficacy and safety of Pueraria mirifica in the treatment of vasomotor symptoms.

Design : Open-label study.

Setting : Hat Yai Regional Hospital, Thailand.

Subjects : Pre and postmenopasusal women with vasomotor symptoms, such as hot flushes and night sweats. Other unpleasant symptoms, urogenital and psychological symptoms, were also evaluated.

Material and Method: Patients were enrolled voluntarily and randomly received 50 mg or 100mg of Pueraria mirifica in capsules, once daily for six months.

Results: of the 48 enrolled patients, 11 cases were excluded for failing to complete the initial work-up. Thirty-seven cases were evaluated. 20 of 37 (54.1%) randomly received a dose of 50mg/day of Pueraria mirifica (Group A). and 17 of 37 (45.9%) received 100 mg/day of Pueraria mirifica (Group B). The mean of the modified Greene climacteric scale deceased from 35.6 to 26.6. 17.2 and 15.1 in group A, while group B, declined from 32.6 to 21.0, 14.8 and 13.6 at 1-, 3- and 6-month respectively. The mean serum estradiol, fluctuated from the baseline of 76.6 to 55.4, 56.7, 72.5, 69.2, 114

Title Journal of Natural Products
Author Sunee Chansakaow,1 Tsutomu Ishikawa,*,1, Hiroko Seki,1 Keiko Sekine (nee Yoshizawwa),1, Mineaki Okada,2 and chi yo chaichantipyuth1
Subject Journal of Natural Products
Institute of Research
Date Issue
Keyword
Abstract

Abstract: Miroestrol (1) has been isolated previously as an active principle from “Kwao Keur” (Pueraria mirifica), a rejuvenating folk medicine from Thailand. Reinvestigation using bioassay-guided purification has resulted in the isolation of a new potent phytoestrogen dexymiroestrol (2). The facile acrial oxidation of 2into 1 suggests the possibility that 1 may be an artifact.

In Thailand, the plant “Kwao Keur” is considered to be a rejuvenating drug and has a fascinating history in folk medicine. 1 Although the name “Kwao Keur” had been applied to several species of plants having tuberous roots, it was definitively identified as Pueraria mirifica in 1952, 2 from which an unusual estrogenic phenol miroestrol 1,3 (I) was isolated as and active principle eight years later.4 Although the possible presence of an alternative active component was suggested, 1 there has been no isolation of any other powerful phytoestrogens, with further studies leading instead to the isolation of isoflavones and

Title P3-206 Pueraria mirifica initiative promotes the cellular mechanism of neuronal survival in neuron human neuroblastoma cells
Author Sayan Sawatsri* 1, Wanphen Yamkunthong 1, Neil Sidell 2.
Subject P3-206 Pueraria mirifica initiative promotes the cellular mechanism of neuronal survival in neuron human neuroblastoma cells
Institute of Research
Date Issue
Keyword
Abstract

Background: Pueraria mirifica (PM) or White Klan Keur (Thai herb)has been used as a rejuvenator for long time in aging. It contains a

variety of phytoestrogens that possess the highest estrogenic activity. In molecular research and epidemiological data show that estrogen replacement therapy (ERT) can decrease the risk of developing Alzheimer\'s disease.Interestingly, whether the estrogenic effect of PM can prevent neurotrophic from neurotoxic agents e.g. glutamate, H202, and beta-amyloid25-35 in AD model. What a mechanism of PM that prevents neuronal cell death is. Objectives: The current study investigated the neurotrophic and neuroprotective action of the complex formulation of phytoestrogen from a standardized PM extract compare with 17b-estradiol in AD model in vitro. Methods: Crude PM was extracted by ethanol and standardized by HPLC. Action of standardize PM was pretreated in human neuroblastoma cell line (LA-N5) in complete media of estrogen deprivation condition

with neurotoxic agents; 0.2 mM glutamate, 20 IxM H202, and 8 I~g/ml beta-amyloid25-35 by inhibit cell death. By using inverted microscope,

morphologic and biochemical analyses were conducted in neuroblastoma cultures to determine the neurotrophic and neuroprotective properties of

standardize PM and 17b-estradiol. Using ICI anti-estrogenic activity and estrogen dependent breast cancer cell for demonstrate the inhibition of PM in estrogen receptor pathway in AD model. Results: the results demonstrated that PM significantly decreased neuronal cell death in a time and dose dependent fashion. Results of neuroprotection studies demonstrated that PM and 10-8 M 17b-estradiol induced highly significant neuroprotectionagainst beta-amyldid, hydrogen peroxide, glutamate-induced toxicity. Inhibit action of PM and 17b-estradiol by estrogen antagonist (ICI164,384) after induce with neurotoxic agents that show significantly increase cell death.

Conclusions: PM shows estrogenic activity similar 17b-estradiol and prevents neurotoxic agents for neuronal dead significantly by may pass

estrogen pathway. For clinical application of PM possible to use for intervention in Alzheimer\'s disease and other neurodegenerative disease in

aging in the near future.